9alpha-halogeno-11beta-azido-steroids and methods for their manufacture



United States Patent 3,455,968 9a-HALOGENO-lLB-AZIDO-STEROIDS AND METHODS FOR THEIR MANUFACTURE Hershel L. Herzog, Glen Ridge, and Elliot L. Shapiro, Cedar Grove, N.J., assignors to Schering Corporation, Bloomfield, N.J., a corporation of New Jersey No Drawing. Filed May 15, 1967, Ser. No. 638,606

Int. Cl. C07c 169/22, 169/34; A61k 17/06 US. Cl. 260-349 15 Claims ABSTRACT OF THE DISCLOSURE Novel 3-oxygenated-9a-halogeno-lIB-azido steroids of the androstane and pregnane series and 19-nor analogs thereof, having valuable pharmacological properties, are prepared via reaction of a 3 oXygenated-9(l1)-dehydro steroid with an alkali metal azide and a positive halogen source.

The 9a-halogeno-11B-azido steroids are also prepared via reaction of a 9a-halogeno-1 lfi-tosyloxy steroid with a saturated aqueous methanol solution of an alkali metal azide.

Described specifically are the 9uc-Cl1l0l0- and the 9ocbromo-l IB-azido-derivatives of steroids of the androstane, pregnane series and 19-nor analogs thereof.

Field of the invention This invention relates to novel compositions of matter classified in the art of chemistry as 9a-hal0gen0-ll13-azido steroids and to process for their preparation.

Summary of the invention The invention sought to be patented in its compositionof-matter aspect resides in the concept of a 9a-halogeno- (preferably Qm-ChlOIO- and 9u-bromo-)-1lfl-azido-steroid (preferably of the androstane and pregnane series and the 19-nor analogs thereof), said 9a-halogeno-llflwazidosteroid being further characterized by having an oxygen function (preferably keto) at C-3, and by having a substituent at 0-17 selected from the group consisting of oxygen,

and

A being a member selected from the group consisting of hydrogen, lower alkyl, lower alkenyl, and lower alkinyl; R being a member selected from the group consisting of hydrogen and lower alkanoyl; T being a member selected from the group consisting of hydrogen, hydroxy, and lower alkanoyloxy, and V being a member selected from the group consisting of fluorine, hydrogen, hydroxy,lower alkanoyloxy, lower alkoxycarboxy, and dihydroxyphosphoryloxy.

The physical embodiments of the composition-of-matter aspect of this invention may have saturated A and B rings or may possess one or more nuclear unsaturations such as at C1, C-4, C-5, C-6 and at C-16, and may be further substituted such as at C-2 and/or at C7 by lower alkyl (preferably methyl), at C-6 by a member selected from the group consisting of lower alkyl (preferably methyl) and halogeno (preferably chloro and fluoro), at C-15 by a member selected from the group consisting of halogeno (preferably chloro), hydroxy and lower alkanoyloxy, and at C-l6 by a member selected from the group consisting of lower alkyl (preferably alpha methyl and beta methyl), lower alkylidene (preferably methyl- Patented July 15, 1969 ene), hydroxy, lower alkanoyloxy, and halogeno (preferably chloro).

The 9a-halogeno-llfl-azido-pregnanes of this invention which also possess at least one hydroxyl group at the 17 aand the 21-positions possess cortical activity. In addition, some of these compounds, e.g. the 9a-halogeno-11B-azido- 4 pregnene l7a,2l-diol-3,2 O-diones and the l-dehydro analogs thereof and the 9a-halogeno-1lfi-azido-lh-hydroXy 21 fluoroprogesterones possess anti-inflammatory activity and are used in a similar manner as known corticoids such as cortisone and hydrocortisone.

The halogeno 1lfi-azido-lh-alkyl-17B-hydroxyandrostanes of our invention are anabolic/androgenic agents.

The 9a-halogeno-11fiazido-l7-keto-androstanes, the 9whalogeno 1lfi-azido-17ei-alkanoyloXy-progesterones and the 170: alkenyland the 170: alkinyl-9a-halogeno-llflazido-testosterone derivatives of this invention possess antiandrogenic and progestational activity and exhibit anticonception properties.

The invention sought to be patented in its process aspect resides in the concept of treating a 9(11)-dehydrosteroid (preferably of the androstane and pregnane series and the l9-nor-analogs thereof) with an alkali metal azide (preferably sodium azide) and a positive halogen source (preferably N-chlorosuccinimide or N-bromoacetamide in the presence of acid) whereby is'obtained a physical embodiment of a 9a-halogeno-11fi-azido steroid of the composition-of-matter aspect of this invention.

GENERAL DESCRIPTION OF THE INVENTION Composition of matter aspect wherein X is a halogen having an atomic weight greater than 20 and less than Y is a member selected from the group consisting of hydrogen and lower alkyl; W is a member selected from the group consisting of hydrogen, lower alkyl (preferably u-methyl and fi-methyl), lower alkylidene (preferably methylene), hydroxy and lower alkanoyloxy; and Z is a member selected from the group consisting of oxygen,

OB. ooomv NA, and ("T A being a member selected from the group consisting of hydrogen, lower alkyl, lower alkenyl, and lower alkinyl, R being a member selected from the group consisting of hydrogen and lower alkanoyl, T being a member selected from the group consisting of hydrogen, hydroxy, and lower alkanoyloxy, and V being a member selected from the group consisting of hydrogen, fluoro, iodo, hydroxy, lower alkanoyloxy, lower alkoxycarboxy, and dihydroxyphosphoryloxy.

The physical embodiments of the composition aspect of this invention are not to be construed as limited to the compounds specifically defined by the above minimal for mula. Also included within the inventive concept are derivatives thereof such as compounds of Formula I having also one or more substituents at C-2 (e.g. a-methyl); C-6 (e.g. 6(0: and 18) -chloro), 6(cc and p)-fluoro), 6 and [ED-methyl; C-7 (e.g. 7a-methyl) at 0-15 (e.g. la-chloro) and the like. 1

By lower alkyl are contemplated hydrocarbon radicals having up to four carbon atoms including methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, tert.-butyl.

By lower alkylidene are contemplated exocyclic unsaturated hydrocarbon radicals having up to four carbon atoms including methylene, ethylidene, propylidene,

and n-butylidene.

-By lower alkanoyl are contemplated saturated hydrocarbon carboxylic acid residues having up to 12 carbon atoms including acetyl, propionyl, valeryl, n-butyryl, caproyl and the like.

Included among the compounds of this invention as defined by Formula I are 9a halogeno llfl-azido corticoids (i.e. pregnanes wherein Z is COGH2OH on and the lower al'kanoate esters thereof) such as 90: chloro 11B azido-l6u-methyl-lA-pregnadiene- 17a,2l diol-3,20-dione and the 17-valerate, 17,21-bis- Zis include propionate, 21-phosphate, 21-acetate, 21-valerate, 21-carboethoxylate esters thereof, 1

9o: bromo 1113 azido-lfiat-methyl-1,4-pregnadiene- 17a,21-diol-3,20-dione and the 2l-phosphate and 2l-acetate esters thereof,

90: chloro-1lp-azido-1,4-pregnadiene-17a,2l-diol-3,20- dione and the ZI-acetate ester thereof,

90: chloro 11B azido-1-6fi-methyl-1,4-pregnadiene- 17a,2l-diol-3,20-dione and the 17-valerate, 17,21-dipropionate, 21-phosphate and 21-acetate esters thereof,

60: methyl 9a. chloro-1lfi-azido-lA-pregnadiene- 17a,2l-diol-3,20-dione, the 21-phosphate and 21-acetate esters thereof, 7

6,90; dichloro 11,6 azido-4,6-pregnadiene-17a,21- diol-3,20-dione, the 2l-phosphate and 21-acetate esters and the l-dehydro analogs thereof. 7

The 9a-halogeno (e.g. 9u-Ch10r0 and 9a-bromo)-1-1B- azido-pregnanes of our invention (in particular the 3-keto- 1,4-bisdehydro compounds of Formula I wherein Z is COCHzV which also possess atleast one hydroxyl group at the 170:- and 2l-positions (i.e. T and/or V is hydroxy) possess cortical activity. In addition, some of the foregoing class of compounds, including the 90: halogeno-llfi-azido corticoids such as listed above and the 9a-halogeno-llfiazido lfla-hydroxy-2lrfluoro-progesterones (i.e. 4-preg n'eneswherein Z is (I v I 7 I I -COCH1V being fluo'r o) exhibit anti-inflammatory activity and are used in the same manner as known corticoids such as cortisone and hydrocortisone.

Thus, for example, 9a-chloro-11;8-azido-16a-methyl-1,4-

9wchloro-l lfi-azidoprogesterone,

9a-br0m0-1 1 B-azidoprogesterone,

9a-chloro-1 1B-azido-17u-acetoxyprogesterone,

9a-bromo-1 lfi-azido-17a-acetoxyprogesterone, 1

9op-Ch10IO-1 lfi-azido-16-methylene-17u-acetoxyprogesterone,

6,9a-dichloro-1 1 fl-azido-l 6-methylene-17u-acetoxy-6- dehydro-progesterone, and 1 Included among the 9a-halogeno-1lfi-azido-androstanes of Formula I (i.e. compounds of Formula I wherein Z is oxygen, and

0 R 1.- are such as: I

9a-chloro-1 15 -azido-4-androsten-17,8-ol-3-one,

9 -chloro-11 8-azido-4-androsten-3,17-dione, 9oz-bl'OIl'lO-l 1,6-azido-4-androsten-17,3-01-3 -one,

' 9a-bromo-1lB-azido-4-androsten-3,17-dione,

90c-Chl01'0-1 lfi-azido-17m-methyl-4-androsten-17,8-01-3- one, 9a,-chloro-1 1 fi-azido-l7a-ethyl-4-androsten-17,8-01-3- one, 9oz-Ch10rO-1 lfi-azido-17a-vinyl-4-androsten-17fl-ol-3-one, 9oz-ChlOrO-l lfl-azido-l7u-ethinyl-4-androsten-175-01-3- ..-one and the corresponding 9a-brotmo derivatives.

The 9a-halogeno 115 azido-l7a-lower alkyl-17fl-hydroxy-androstanes of Formula I, e.g. 9a-chloro-11/8-azido- 17a-methyl-4-androstene-17fi-ol-3-one and 9a-bromo-11fiazic lo-17u-methyl-4-androstene-17,8-01-3-one are anabolic agents having a favorable anabolic/androgenic ratio and may 'be used in place of anabolic/ androgenic agents used in known pharmaceutical preparations, the dose administered varying with the nature and severity of the patients symptoms being treated. V ,The 9a-halogeno-11,8-azido-17-keto-androstanes (e.g. 9achloro-llfi-azidoA-androsten-ll7-dione) the 9a-halogene-1l,B-azido-l7a-alkenyl-l7fi-hydroxy-androstanes and fiuoromethylethinyl) e.g. 9a-chl0ro-l1,8-azido-17a-ethinyl the halogeno-ll S-azido-17a-alkinyl-l7 8-hydroXy-androstanes (e.g. 9a-chloro-1lfi-azido-17u-vinyl-4-androsten-l7/3-ol-3-one and 9a-chlor0-11fl-azido-17a-ethinyl-41 androsten-17fi-ol-3-onej and the 9u-halogeno-1lfi-azido- 17 -alkanoyloxy-progesterones (e.g. 9a-chloro-1lp-azido- 1 7a-acetoxy-progesterone and 6,9a-dichl0ro-1lfi-azido-lfimethylene-l7a-acetoxy-4,6-pregnadiene-3,20-dione) possess to some extentanti-androgenic and progestational activity and exhibit anti-conceptional properties.

Those compounds wherein Z represents (the alkinyl -being eth inyl, propinyl, halogenoethinyl, tri- 4-androsten-l7B-ol-3-one, demonstrate anti-conception properties. They are also anti-androgenic agents as evi-. denced by their anti-gonadal hormonal properties such as demonstrated by their ability to depress secondary sex structures in both male and female animals, this making them useful for the treatment of prostatic hypertrophy in dogs. Also of particular use as anti-androgenic agents are the 6-chloro-9a-halogeno-1 lfi-azido-6-dehydro-progesterones, in particular 6,9a-dichloro-lIB-azido-lG-methylenel7a-acetoxy-6-dehydro-progesterone.

The 90: halogeno-l lfi-azido-l7u-alkenyl-17fi-hydroxyandrostanes, e.g. 9a-chloro-11p-azido-17a-vinyl-4-androsten-17fi-ol-3-one are to some extent progestational making them of use, for example, in delaying the onset of heat in dogs and cats.

The 9a-halogeno-1lfi-azido-17m-alkanoyloxy-progesterones, in particular 9a-chloro-1LB-azido-l7a-acetoxy-progesterone and the 16-methyleneand the 6a-chloro-16- methylene derivatives thereof, exhibit progestational activity and are used in similar manner and for the same disorders as known progestins, e.g. progesterone, ethisterone, and the like.

In general, the pharmaceutical formulations of our 90:- halogeno-l1/8-azido-steroids are prepared and are administered utilizing procedures known in the art. For example, the physiologically active compounds of this invention, such as described above, may be administered orally or parenterally, by incorporating a therapeutic dosage in conventional pharmaceutical form such as tablets, capsules, elixirs, suspensions, solutions, or the like. They can be administered in admixture with pharmaceutical excipients which are edible and which are chemically inert to the aforementioned 9a-halogeno-llB-azido-steroids exemplified by cornstarch, lactose, sucrose, gum arabic usually in admixture 'with an additive such as magnesium stearate, talc, and the like. Other compositions may be used such as fine powders or granules of 9a-halogeno-lltt-azido steroids, which compositions may contain diluents and dispersing and surface active agents, and may be presented in a syrup, or in non-aqueous suspensions, in aqueous suspensions or in an oil.

PREFERRED EMBODIMENTS OF COMPOSITION OF MATTER ASPECT Of the foregoing classes of compounds of the composition of matter aspect of this invention, the preferred embodiment are the 9a-halogeno-llfi-azido-lA-pregnadiene-17a,21-diO1-3,20-di0116s and their esters which exhibit anti-inflammatory activity. Of particular interest are the following compounds:

90: chloro-l 1fi-azido-la-methyl-l,4-pregnadiene-17a, 2l-diol-3,20-dione and the 17-valerate, 17,21-dipropionate, 21-phosphate, ZI-acetate and the 2l-carbethoxy1ate esters thereof,

9a chloro-llfi-azido-lfiB-methyl-l,4-pregnadiene-l7a, 21-diol-3,20-dione and the 17-valerate, 17,21-dipropionate, 21-acetate and the 2l-phosphate thereof,

9u-chloro 11,3 azido-l,4-pregnadience-l7a,2l-diol-3, 20-dione and the Zl-acetate ester thereof, and

9a chloro 115 azido-4-pregnene-17u,21-di0l-3,20- dione and the 21-acetate esters thereof.

GENERAL DESCRIPTION OF PROCESS ASPECT OF INVENTION One method of preparing the 9a-Ch10I'0-1 1,8-azido and the 9a-bromo-l lfi-azido-steroids of Formula I is by the process aspect of this invention whereby a 9(l1)-dehydro steroid preferably of the androstane and pregnane series, e.g. 16c: methyl-1,4,9(11)-pregnatriene-17a,21-diol-3,20- dione 21-acetate, in a lower alkanoic acid (e.g. acetic) is treated with an alkali metal azide (e.g. sodium azide) and a positive halogen donor (e.g. N-chlorosuccinimide or N-bromoacetamide) whereby is obtained the corresponding Qa-ChiOI'O-llfi-fllidO- or 9a-bromo-11B-azido steroids, i.e. the physical embodiments of the composition-of-matter aspect of this invention, e.g. 9u-chloro- 11B azido-l6a-methyl-1,4-pregnadiene-170:,21-diol,3,20- dione 21-acetate and 9a-bromo-11p-azido-16u-methyl- 6 1,4-pregnadiene-l7a,21-diol-3,20-dione 21-acetate respectively.

Halogenating agents which are sources of positive chlorine and bromine and which are useful in our process are such as molecular chlorine and bromine, N-chlorosuccinimide and N-bromosuccinimide, N-bromoacetamide, dimethyl-N,N-dibromohydantoin, and tribromophenolbromide.

The 9(11)-dehydro-steroids utilized as starting compounds are known in the art and may be prepared by known procedures such as described for example, in US. Patents Nos. 2,894,963; 3,009,938; 3,049,544; 3,009,927; 3,087,938; 3,009,930; 3,009,928; 3,009,929; 3,009,931; 3,131,200; 3,009,932; 3,290,338; 3,032,564; 3,026,339; 3,009,933; and 3,312,692. In preparing 9a halogeno 1113 azido steroids of Formula I which are substituted such as at C-2, C-6, C7, C15 and C-l6 or which have additional unsaturations such as at C-1 and/or C6, it is advantageous when utilizing the process aspect of this invention that all the desired substituents and/or unsaturations be present in the 9(1l)-dehydro steroid prior to reaction with sodium azide and the source of positive halogen. It is also desirable to protect any primary or secondary alcohol groups present in the molecule, for example by esterification thereof, prior to reaction With sodium azide and positive bromine or chlorine. Thus, 6,9a-dichloro- 11B azido-l6-methylene-l7a-acetoxy-6-dehydro progesterone is preferably prepared by our process by reaction of 6 chloro-l6-methylene-17a-acetoxy-6,9(11)-bis-dehydroprogesterone in acetic acid with sodium azide and N- chlorosuccinimide.

The process of this invention is conveniently carried out in aqueous lower alkanoic acids such as acetic acid, propionic acid, N-butyric acid and the like. The lower alkanoic acid thus serves a dual role as solvent for the steroid and as a source of hydrogen ion for the positive halogen donor reagent. Our process is usually carried out at room temperature.

Generally when carrying out the physical embodiment of the process aspect of our invention a 9(11)-dehydrosteroid, e.g. 6a,16 x-dimethyl-1,4,9(l1)-pregnatriene-l7a, 21-diol-3,20-dione 21-acetate is dissolved in acetic acid to which is added large molar excesses of sodium azide in aqueous acetic acid (usually saturated solutions thereof) to which is added at least a molar equivalent of the positive halogen donor and usually about two or three molar equivalents (e.g. at least one mole N-chlorosuccinimide or at least one mole N-bromoacetamide per mole of 9(11)-dehydro steroid. The reaction is usually continued at room temperature until the mixture tests negative to starch iodide paper. When an excess of halogenating agent is used, the starch iodide test is eliminated and the reaction continued until complete as evidenced by chromatographic analysis (to determine the absence of starting material). The 9u-halogeno-1IB-azido-steroids produced by our process (e.g. 6oz,16ot-diIIl6thYl-9a-Ch10l'0- 1lfl-azido-1,4-pregnadiene-l7or,21-dio1-3,20-dione 2l-acetate or 6a,16a-dimethyl-9ot-bromo-1lfl-azido-lA-pregnadiene-17a,21-diol-3,20-dione 2l-acetate respectively) is conveniently isolated by pouring the reaction mixture into water and separating the precipitate by filtration or extraction with a suitable solvent. Purification is effected by techniques well known in the art such as by recrystallization, chromatography on adsorbants such as Florisil or silica gel, thin layer chromatography and the like.

The preferred mode of carrying out our process is that utilizing aqueous acetic acid as solvent, sodium azide and N-chlorosuccinimide as reagent when preparing the 9u-chloro-derivatives, or N-bromoacetamide as reagent when preparing the 9a-bromo derivatives.

Second process for preparing 9a-halogeno- 11 fl-azido-st'eroids The above represents the process aspect of this invention whereby are prepared the physical embodiments of the 9tx-halogeno-1lfl-azido-steroids of the composition of matter aspect of this invention. Another method of preparing the 9u-halogeno-llfi-azido-steroids of Formula I is by means of a replacement reaction described in copending application Ser. No. 551,926 of Hershel L. Herzog et al., filed May 23, 1966. By this process, a 9mhalogeno-llfi-sulfonyloxy (preferably tosyloxy)-steroid in a polar solvent (preferably a hydroxylic solvent), advantageously kept at a pH in the range of from 6 to 7.5, is treated with a saturated solution of alkali metal azide (preferably sodium azide), whereby the llfi-sulfonyloxy function (e.g. tosyloxy) is replaced by the azido function with concomitant formation of a sulfonic acid (e.g.

p-toluenesulfonic acid) and the corresponding 9u-halogeno-l l}3azido-steroid.

Hydroxylic solvents advantageously used in this process include Water, lower alkanols (preferably methanol), phenols, and hydrocarbon carboxylic acids, preferably lower alkanoic acids such as acetic mixtures of the foregoing.

Sulfonyloxy radicals at C11 which may be replaced by azido according to this process include alkylsulfonyloxy radicals, e.g. methanesulfonyloxy (i.e. mesyloxy) and ethanesulfonyloxy; arylsulfonyloxy radicals, e.g. benzenesulfonyloxy and naphthalene-[3-sulfonyloxy; aralkylsulfonyloxy radicals, e.g. p-toluenesulfonyl (i.e. tosyloxy), ortho-toluenesulfonyloxy, meta-toluenesulfonyloxy, 3,4-xylenesulfonyloxy and dodecylbenzenesulfonyloxy.

When carrying out this process utilizing as starting compound of 9u-halogeno llB-tosyloxy-Zl-hydroxy steroid, the 21-hydroxy group is preferably esterified, such as by a 21-phosphate, 2l-carbethoxylate, or a 21-acetate ester.

In general, when carrying out this process, a solution of the 9a-chloro (or bromo)-llfl-sulfonyloxy (e.g. tosyloxy)-steroid together with a saturated aqueous methanol solution of sodium azide are stirred together at moderate temperatures (usually in the range of from about 30 to 75 C.) until the reaction is complete as evidenced by chromatographic analysis (to determine the absence of starting material) as well as other techniques known in the art. Isolation of the resulting 9a-halogeno-11 3-aZido steroid is then effected according to procedures Well known in the art.

In a preferred mode of the replacement process for preparing a 9a-halogeno-ll 8-azido-steroid, a 9oc-Chl010- llfl-tosyloxy-steroid (e.g. 9a-chloro-16a-methylprednis olone 11-tosylate21-acetate) is added to a saturated solution of sodium azide in aqueous methanol (e.g. 1:4 water-methanol) (said solution having been titrated to about pH 6 with acetic acid), and said reaction solution is stirred at about 60 C. for about 24 hours, whereby there is formed 9a-chloro-l1;3-azido-16a-methyl-1,4- pregnadiene-17a,21-diol 3,20 dione 2l-acetate which is isolated and purified utilizing techniques known in the art.

In this foregoing replacement reaction, it is advantageous to bufier the reagent solutions thereof to a pH in the range of from about 4 to about pH 7.5 prior to addition of the 9a-halogeno-11fi-tosyloxy steroid in order to minimize undesirable side reactions such as that between halogeno groups and strong base.

In general, the replacement process proceeds at a faster rate when carried out in the presence of water; thus, when replacing the llfi-tosyloxy group at 6-11 an azide, it is advantageous to utilize aqueous methanol as solvent rather than anhydrous methanol. With the presence of water in the reaction mixture, in addition to effecting replacement of the llfi-tosyloxy group by azide, some llfl-hydroxy steroid is formed which is easily separated from the resulting llB-azido derivative via chromatographic techniques.

The 9ot-halogeno-1lfi-sulfonyloxy-steroids, necessary and propionic, and

v 8 intermediates in; thereplacement process, preferably possess all the substituents and/or unsaturations'desired in the 9a-halogeno-1lfi-azido final product.

The 9a-halogeno-l lfl-sulfonyloxy interrnediates are prepared via procedures known in the art from the corresponding 9(11)-dehydro steroid by reaction with ptoluenesulfonic acid in pyridine together with N-bromosuccinimide or N-chlorosuccinimide,for example, whereby are prepared the corresponding 9a-bromo-1lfi-tosyloxy and the 9a-chloro-llfl-tosyloxy steroids, respectively. Some typical intermediates of this group are:

9a-chloro-1 lfi-p-toluenesulfonyloxy-1,4-pregnadiene- 17a,21-diol-3,20-dione 2l-cathylate,

9a-chloro-1 IB-tosyloxy-l,4-androstadien'lf7 8-ol-3-one l7-propionate 9a-chloro-1 lp-tosyloxy-19-nor-4-androstene-3,17-dione.

The following examples exemplify the preferred modes of preparing the physical embodiments of the composition aspects of our invention. It is to be understood that the examples are .for purposes of illustration and are not to be construed as limiting the invention; Obvious equivalents including those set forth above will be apparen to one skilled in the art. v

7 EXAMPLE .1 9a-chlOl'O-l lfi-azido-l 6a-methyl-1,4-pregnadiene- 17a,21-diOl3,20-dl0t18 v A. 9oz-Chl0IO-l 1,8-azido-16a-methyl-L4 pregnadie'ne- 17a,2l-diol-3,20-dione 21-acetate (preparation via direct addition method).Stir a solution of 4 g. of 16u-methyl- 1,4,9( 1 1 )-pregnatriene-l7ot-2l-diol-3,2-0-dione 2l-acetate and 10 g. of sodium azide in 40 ml. of acetic acid and 10 ml. of water at room temperature and add 1.3 g. of N-chlorosuccinimide' portion-wise over a one hour period, the dilute the reaction mixture with 20 ml. of acetic acid and add a second 10 g. portion of sodium azide. Stir another hour at room temperature whileadding an additional 1.3 g. of N-chlorosuccinimide portionwise. Stir until the reaction mixture tests negative to starch iodide paper (which is usually at the end of the 12-hour period), then pour the reaction mixture into' 300 ml. of cold water and filter the resultant precipitate comprising 9u-chloro-11B-azido 16u-methyl-1,4-pregnadiene-17a-2 l-diol-3,20-dione 21-ace-tate.

Purify by first chromatographing on Florisil eluting with increasing amount of ether in hexane. Combine the eluates of the 50100% ether-in-hexane mixtures and concentrate to a residue. Further purify this residue via preparative thin-layer chromatography on silica gel utilizing as solvent ethylacetate/choroform 1:3. Extract with chloroform and chloro-azide product is determined by infrared and ultraviolet spectra. Recrystallize the residue of the chloroform extracts with ethyl acetate, M.P. 246- 247 C. +177.8 (dioxane).

B. 9oz chloro 11,8 azido 16cc methyl 1,4 pregnadiene 170;,21 diol 3,20 dione-To a solution of 10 ml. of methanol and 0.6 m1. of chloroform containing 0.365 ml. of perchloric acid add 0.18, g. of 9achloro-l lfi-azido-l6a-methyl-1,4-pregnadiene-17a,21-dio1- 3,20 dione ZI-ace'tate. Stir the reaction :mixture at room temperature about 18 hours then pour into 100 ml. of water. Pass nitrogen into the reaction mixture for 45 minutes. Filter the resultant precipitate comprising 9w chloro 11B azido 16a methyl 1,4 pregnadiene1 17a,2l-diol-3,20-dione. V V

EXAMPLE 2 I -.chloro 11 3 azido 16cc methyl 1,4 pregnadi- CHE-1704,2 1-dl0l 3,20-dione 21-acetate (via replacement 16a-methyl-1,4-pregnadiene-l7a,21-diol-3,20-dione 21- acetate) I A. 9a-chloro1lfi-tosyloxy-16a-methyl-1,4-pregnadiene- 17u,21-diol-3,20-dione 21-acetate.To a solution of 16mmethyl-l,4,9(11)-pregnatriene-l7u,2l-diol-3,20-dione 21- acetate (6.0 g.) in 600 ml. of pyridine, add 8 g. of sodium p-toluene sulfonate and 30 g. of p-toluenesulfonic acid monohydrate. Concentrate the mixture to a volume of 300 ml., then add 4 g. of N-chlorosuccinimide and stir at room temperature for 24 hours. Pour the reaction mixture into 3 liters of cold 10%, aqueous sulfuric acid. Filter and air dry the resultant precipitate comprising 9a-chloro- 11,8 tosyloxy 16a. methyl 1,4 pregnadiene 17cc, 2l-diol-3,20-dione 21-acetate. Purify via chromatography on silica gel eluting with 15% ethyl acetate in chloroform. Evaporate the combined eluates to a residue, then crystallize the residue from acetone-hexane; M.P. 203- 206 C., [01],; +71.2 (dioxane),

xgitxhanol 30 mp :19,.500)

B. 911 chloro-115 azido 16oz methyl 1,4 pregnadiene-l7a,21-diol-3,20-dione 21-acetate.To a solution of 100 g. of sodium azide in one liter of water and 2.5 liters of methanol, add acetic acid until the solution is at pH 6.0. Add 2 g. of 9a-chloro-l1B-t0syloxy-l6a-methyll,4-pregnadiene-l7a,2l-diol-3,20-dione 21-acetate and stir at 60 C. for 24 hours. Add 3 liters of water to the reaction mixture and extract with methylene chloride. Wash the methylene chloride extracts with water, dry over magnesium sulfate and concentrate to a residue comprising 901 chloro 11B azido 16a methyl 1,4 pregnadiene- 170:,21-(1101 3,20-dione 21-acetate. Purify via preparative thin layer chromatography on silica gel utilizing as solvent ethyl acetate-cyclohexane (2:3). Extract with chloroform the chloro-azide product as determined by spectroscopic analysis, then recrystallize the residue of the chloroform extracts from ethyl acetate; M.P. 246-247" C., [11],; +177.8 (dioxane),

aitlin-n01 my EXAMPLE 3 9oz-ChlO1'0-1 lfi-tosyloxy-l6a-methyl-1,4-pregnadiene- 17cc,2l-di01-3,20-di01'16 2 l-phosphate A. l6a-methyl-1,4,9(l1)=pregnatriene-l7a,21-dio1-3,20 dione 21-methanesu1fonate.To a solution of 5 g. of 160:- methyl-l,4,9(11)-pregnatriene-17a,21-diol in 50 ml. of pyridine cooled to -20 C., add dropwise 5 ml. of methanesulfonyl chloride. Stir at -20 C. for 30 minutes then pour into water and stir for 2 hours longer at room temperature. Filter and dry the resultant precipitate comprising 16!; methyl 1,4,9(11) pregnatriene 1711,21 diol- 3,20-dione ZI-methane sulfonate. Purify by crystallizing from acetone-hexane (after charcoal treatment of the solution), M.P. 179-182 C.

B. 160: methyl 21 iodo l,4,9(11) pregnatrienel7a-ol-3,20-dione.To 3.5 g. of l6u-methyl-1,4,9(l1)- pregnatriene-17a,2l-diol-3,20-dione 2l-methanesulfonate in 52.5 ml. of acetone add 3.5 g. of sodium iodide. Heat the reaction mixture at reflux temperature for 20 minutes then pour into 500 ml. of water, filter and air dry the resultant precipitate comprising 16a-methyl-21-iodo-L4, 9(1l)-pregnatrien-17a-ol-3,20-dione. Purify by crystallization from acetone-hexane, M.P. 143l44 C., +56 (dioxane),

Aggihannl 239 mp =16,400)

C. 16oz methyl 1,4,9(l1) pregnatriene 1701,21- diol-3,20-dione 21-phosphate.To 43 ml. of methanol at room temperature add dropwise with stirring 24 ml. of 85% aqueous phosphoric acid. Add cautiously 75.4 ml. of triethylamine followed by 12.7 g. of 16a-methyl-21- iodo-1,4,9(1l)-preguatrien-17a-ol-3,20-dione. Warm the reaction mixture on a steam bath for 30 minutes then with stirring pour into water (260 ml.) containing concentrated hydrochloric acid (72.5 ml.). Filter the resultant precipitate comprising 16a-methyl-l,4,9(1l)-pregnatriene-17a,2l-diol-3,20-dione 2l-phosphate. Purify by crystallization from aqueous acetone.

D. chloro 11B tosyloxy 16oz methyl 1,4- pregnadiene-17a,21-diol 3,20-dione ZI-phosphate. Dissolve 10 g. of p-toluenesulfonic acid monohydrate in 200 ml. of pyridine and concentrate in vacuo to a volume of ml. Add 2.733 g. of sodium p-toluenesulfonate, 1.371 g. of N-chlorosuccinimide and immediately 2 g. of 16u-methyl-1,4,9( 1 l )pregnatriene-17u,2l-diol-3,30-dione 21-phosphate. Stir under an atmosphere of nitrogen for 18 hours. Pour the reaction mixture into 1 liter of 10% aqueous sulfonic acid then filter and air dry the resultant precipitate comprising 9a-chloro-11B-tosyloxy-16a-methyl-l,4-pregnadiene-l7a,21-diol-3,20-dione 2l-phosphate.

Purify by crystallization from methanohethyl acetate, M.P. 182-185 C. (dec.).

EXAMPLE 4 9a chloro 11p azido 16oz methyl 1,4 pregnadiene- 17u,2l-dl01-3,20-d10118 2l-acetate (via replacement of the 11 ,B-tosyloxy group in 9oc-Ch101O-1Ifii-tOSYlOXY-lGumethyl-1,4-pregnadiene-17a,21-diol-3,20-dione 21-phosphate) A. 90: chloro 1113 azido 16a methyl 1,4 pregnadi61'1e-17oz,21-diO1-3,20-di0I1e 21-phosphate.-Stir a reaction mixture of 1.6 g. of 9a-chloro-l lfl-tosyloxy-lfia-methyl-l,4- pregnadiene l7a,21-diol-3,20-dione 2l-phosphate and 80 g. of sodium azide in 400 m1. of water for 2 days. Pour the reaction mixture into 8 liters of water, acidify with ml. of concentrated hydrochloric acid, and extract with isoamyl-alcohol. Wash the combined isoamylalcohol extracts with water then evaporate to a residue comprising 911 chloro-llfi-azido-l6a-methy1-1,4-pregnadiene-l7a,2l-diol-3,20-dione 21-phosphate which is used without further purification in procedure 4B immediately following.

B. 911 chloro 11/3 azido 16cc methyl 1,4-pregnadiene-l701,21-diol-3,20-dione.-To 300 mg. of Qua-6111010- 11/3 azido 16a methyl 1,4 pregnadiene 1704,21- dio1-3,20-dione 21-phosphate (as prepared in Example 4A) dissolved in 6 ml. of a one molar aqueous solution of trishydroxymethylaminomethane titrated to pH 8 with hydrochloric acid, add 0.3 ml. of bacterial alkaline phosphatase (Worthington Biochemical Corporation, Freehold, N.J.chromatographically purified grade). Maintain the reaction mixture at 37 C. for 20 minutes, add water and extract with methylene chloride. Combine the methylene chloride extracts, wash with water, dry over sodium sulfate and evaporate to a residue comprising 90:- chloro 115 azido 16oz methyl 1,4 pregnadiene- 17a,2l-diol-3,20-dione. Purify by preparative thin-layer chromatography on silica gel utilizing as solvent ethyl acetate-cyclohexane (1:1). From the preparative plate extract the more polar product with acetone. Concentrate the acetone extract and crystallize the resultant residue from acetone-hexane to yield purified 9a-chloro-1lfl-azido- 16a-methyl-1,4-pregnadiene-17a,21-diol-3,20-di0ne, M.P. -488 C. (deC.),[Ot] +155.8 (dioxane),

.90: chloro 11B azido 16a methyl 1,4 pregnadiene- 17a,21-diol-3,20-dione 21-acetate. Purify by crystallization from ethyl acetate, M.P. 246-247 C.

EXAMPLE 5 9a chloro 11B azido 7 16a methyl 1,4 pregnadiener l7a,21-diol-3,20-dione 21-cathylate A. 90: chloro 11/3 tosyloxy 16cc methyl 1,4- pregnadiene-l7a,21-diol-3,20-dione 2l-cathylate. The requisite intermediate 16u-methyl-1,4,9 1 1 pregnatriene- 17a,2l-di0l-3,20-di0116 21-cathylate is prepared from 160:- methyl l,4,9(11) pregnatriene 17a,21 diol 3,20- dione utilizing known procedures by treatment with ethylchlorocarbonate' in pyridine at room temperature.

Dissolve 100 g. of p-toluenesulfonic acid monohydrate and 27.33 g. of sodium p-toluenesulfonate in one liter of pyridine and concentrate this solution in vacuo to about 500 ml. maintaining the temperature at about 40 C. Cool to room temperature and add 13.17 g. of recrystallized N-chlorosuccinimide followed immediately with 20 g. of 16m methyl 1,4,9(11) pregnatriene 1711,21 diol- 3,20-dione 2l-cathylate (prepared as described above).

Stir the reaction mixture at room temperature for 20 hours, then pour into liters of an ice/water mixture containing one liter of concentrated sulfuric acid. Filter the resultant precipitate, wash to neutrality with water and air dry at 60 C. to give 9a-Chl0I'0-1lfi-IOSYIOXY-lfiotmethyl 1,4 pregnadiene 170:,21 diol 3,20 dione 21-cathylate. Purify by chromatography on silica gel eluting with l0l5% ethyl acetate in chloroform. Combine the early, like fractions and concentrate to a residue. Crystallize this residue from acetone-hexane to give 9u-chloro- 11B tosyloxy 16oz methyl 1,4 pregnadiene 17a,21- diol-3,20-dione 21-cathylate; M.P. 201-203" C. [ab +65 (dioxane),

M332) 231 mu (e=26,600)

B. 9:: chloro 11/3 azido 16a methyl 1,4 pregnadiene 170:,21 diol 3,20 dione 21 cathylate.-Add acetic acid to a solution of 150 g. of sodium azide in 1.5 liters of water and 1.5 liters of methanol until the pH of the solution is 6.6. Add 3 g. of 9a-chloro-llfl-tosyloxy- 28 to obtain 9a-chloro-llfi-azido-17a-hydroxy-nor-progesterone.

I EXAMPLE 7, 9achloro+1lfi-azido-1,4-androstadien-17p-ol-3-one A. 9a chloro-1lfi-tosyloxye1,4-androstadien-175-01-3- one.Diss'olve '40 g. of p-toluenesulfonic acid monohydrate and 7 gm. of'sodium p-toluene sulfonate in 600 ml. of pyridine. Then concentrate the solution in vacuo to about 300 ml. At roomtemperature add 3 gm. of 1,4,9 (1l)-androstatrien-17;8ol-3-one followed immediately by 1.9 gm. of recrystallized N-chlorosuccinimide. Stir the reaction mixture at room temperature for 20 hours, then followed'by crystallization from acetone-hexane to give 9oz chloro 11 8 azido 16a methyl 1,4 pregnadiene- 17a,21-diol-3,20-dione 21-cathylate; M.P. 220- 223 C. [0:1 +168.4 (dioxane),

methanol mill.

A. 9a chloro-1lfi-tosyloxy-lh-hydroxy-l9-nor-proges terone.In a manner similar to that described in Example 5A, dissolve 48 g. of p-toluenes'ulfonic acid monohydrate and 9.7 g. of sodium p-toluenesulfonate in 600 ml. pyridine and concentrate the solution in vacuo'to a volume of 400 ml. Add 4 g. of 17ot-hydroxy-l9-nor-4,9(11)-progesterone followed immediately by 1.9 g. of recrystallized N-chlorosuccinimide. Stir at room temperature for 18 hours. Isolate and purify the resultant product in a manner similar 'to that described in Example 5A ,to give 9a-chloro-11fltosyloxy-l7a-hydroxy-19-nor-progesterone.

B. 9a chloro 11 3 azido-17a hydroxy-1'9;nor-progesterone-.In a manner similar to that described in Example 2B, treat 9a-chloro-1lfi-tosyloxy-17a-hydroxy-19-norprogesterone with sodium azide in methanol-water at pH 6.0 at 60 C. for 24 hours. Isolate and purify the resultant product in a manner similar to that described in Example centrated sulfuric acid. Filter, wash to neutrality with water and air dry at' 60 C., the resultant precipitate comprising 9o:- chloro-1lfl-tosyloxy-l,4-androstadien-175-01-3- one. Purify via chromatography on silica gel, eluting with 10: 15% ethyl acetate in chloroform. Concentrate the combined eluates and crystallize the resultant residue from acetone-hexane.

B. .chloro 1IB-azido-1,4-androstadien-1719-01-3 0ne.'-In a manner similar to that described in Example 2B, treat 9a-chloro-l lit-tosyloxy-1,4-androstadien-17B-ol- 3-one with sodium azide in methanol-water at pH 6.0 for 24 hoursat 60 C(Isolate and purify the resultant product to obtain 9oc-ChlOIO-l LB-azido-1,4-androstadien-17,8-01- 3-one.

EXAMPLE 8 9a-chloro-l lp-azido-l9-nor-4-androstene-3,l7-dione A. 9m "chloro-1lp-tosyloxy-l9-nor-4-androstene-3,17- dione.'Dis'solve 70 g. of p-toluenesul-fonic acid monohydrate and 19 g. of sodium p-toluene sulfonate in 1 liter of pyridine, then concentrate the solution to a volume'of 500 ml. Add 5 g. of 19-nor-4,9(l1)-androstadiene-3,17-dione followed immediately by 2.3 g. of recrystallized N-chlorosuccinimide and stir at room temperature for 18 hours. Pour the reaction mixture into 4 liters of iced water containing 400 ml. of concentrated sulfuric acid. Filter the resultant precipitate, Wash to neutrality with water and air dry to yield 9a-chloro-1llit-tosyloxy-19-nor-4-androstene-3,17-dione. Purify via chromatography on silica gel eluting with 10:15% ethyl acetate in chloroform. Concentrate the combined eluates and crystallize the resultant residue from acetone-hexane.

B. 904 chloro 11B azido-l9-nor-4-androstene-3,17- dione.In a manner similar to that described in Example 2B, treat 90; chloro-1lfl-tosyloxy-19-nor-4-androstene-3, 17-dione with sodium azide in methanol-water at pH 6.0 for 24 hours at 60 C. Isolate and purify the resultant product in a manner similar to that described to obtain 9a-chloro-1 lfl-azido-l 9-nor-4-androstene-3, 17-dione.

EXAMPLE 9 9u-chloro-1lfi-azido-1,4-pregnadiene-16a,l7u,2l-triol- 3,20-dione 16,21-diacetate A. 90: chloro 11 it-tosyloxy-1,4-pregnadiene-16a,17a, 21-triol-3,20-dione 16,21-diacetate.In a manner similar to that described in Example 5A treat 1,4,9(11)-pregnatriene-16a,l7u,21-triol-3,20-dione 16,21-diaceate with ptoluenesulfonic acid in pyridine followed by N-chlorosuccinimide. Isolate and purify the resultant product in a manner similar to that described to obtain 9a-chloro-l1,B tosyloxy 1,4-pregnadiene-l6a,17a,21-triol-3,20-di0ne 16, 2l-diacetate. v I

B. 904 chloro'-, 11,B-azido 1,4-pregnadiene-16a,17u,2ltriol-3,20 -dione l6,2l-diacetate.-In a manner similar to that described in Example 5B treat 9u-chloro--/3-tosyloxy- 1,4 pregnadiene-l6a,17a,21-triol-3,20-dione 16,21-diacetate with sodium azide in aqueous methanol. Isolate and purify the resultant product in a manner similar to that described in Example 5B to obtain 9a-chloro-llfl-azido- 1,4 'pregnadiene-16a,l7a,21 triol-3,20-dione 16,21-diacetate.

13 EXAMPLE 10 9a-chloro- 1 1B-tosyloxy-4-pregnene-17u-21-diol-3,20-dione 21-phosphate intermediates A. 9( 1 1) dehydropregnane-2l-methanesulfonate intermediates.In a manner similar to that described in Example 3A treat each of the following 2 l-hydroxypregnames in pyridine with methanesulfonyl chloride:

(1 1,4,9(11)-pregnatriene-17u,21-dio1-3 ,ZO-dione (2) l6,6-methyl-1,4,9(1 1)-pregnatriene-17a,21-dio1- 3,20-dione (3 6amethyl-1,4,9(11)-pregnatriene-17a,21-diol- 3,20-dione (4) 2ix-methyl-4,9 l l pregnadiene-17a,2 l-diol- 3,20-dine (5) 16a-ethyl-4,9( l1 )-pregnadiene-17a,21-diol-3,20-

dione (6) 6u-methyl-4,9 l1)-pregnadiene-l7a,21-diol-3,20-

dione Isolate and purify the resultant respective products in a manner similar to that described in Example 3A to obtain:

(1 1,4,9 l l -pregn atriene-l7a,21-diol-3,20-dione 21-fethanesulfonate (2) 16B-methyl-1,4,9(11)-pregnatriene-17a,2l-diol- 3,20-dione 2l-methanesulfonate (3 6u-methyl-1,4,9(1l)-pregnatriene-l7a,2l-diol- 3,20-dione 21-methanesulfonate (4) 2a-methyl-4,9(1l)-pregnadiene-17u,2l-diol-3 ,20-

dione Zl-methanesulfonate (5) 16a-ethyl-4,9(ll)-pregnadiene-17a,21-diol-3 ,20-

dione 2l-methanesulfonate (6) 6tz-methyl-4,9(11)-pregnadiene-l7a,2l-diol-3 ,20-

dione 21-methanesu1fonate B. 21-iodo-9 11)-dehydropregnane intermediates.In a manner similar to that described in Example 3B treat each of the pregnane 21-methanesulfonate esters prepared in Example 10A above (i.e. Products 10A-1-1OA-6) with sodium iodide in acetone. Isolate and purify the resultant respective products in a manner similar to that described above to obtain respectively:

(1) 2 l-iodo- 1,4,9 (1 1 -pregnatriene-17a-ol-3,20-dione (2) l6 3-methyl-2l-iodo-l,4,9(l1)-pregnatriene-17aol-3,20-dione (3 6a-methyl 21-iodo-l,-4,9 (11)-pregnatriene17uol-3,20-dione (4) Za-methyl-Z l-iodo-4,9( l l -pregnadiene-l7a-ol- 3,20-dione (5) '16a-ethyl-2 l-iodo-4,9 1 l -pregnadiene-17a-ol- 3,20-dione V (6) 6a-methyl-2 1-iodo-4,9 1 l pregnadiene-l7a-ol- 3,20-dione C. 9,11 dehydropregnane 21 phosphate intermedi ates.In a manner similar to that described in Example D. 9.; chloro 11B tosyloxypregnane 2l phosphate intermediates.--In a manner similar to that described in Example 3D treat each of the 9,11-dehydropregnane 21- phosphate intermediates 3C-13C-6 with p-toluenesulfonic acid in pyridine followed by sodium p-toluenesulfonate and N-chlorosuccinimide. Isolate and purify the resultant respective product in a manner similar to that described to obtain respectively:

(1) 9a-Chl0l'O-1 lfi-tosyloxy-l ,4-pregnadiene-l7a,21-

diol-3,20-dione 21-phosphate (2) 9a-chloro-l lB-tosyloxy-lGB-methyl-1,4-pregnadiene- 17a,21-diol-3 ,20-dione 21-phosphate (3) 6a-methyl-9nt-chloro-1 lfi-tosyloxy-l,4-pregnadiene- 17u,2l-diol-3,20-dione 2l-phosphate (4) 2a-methyl-9 a-chlorol l B-tosyloxy-4-pregnene- 17a,2l-diol3,20-dione 21-phosphate (5 9a-chloro-1lfi-tosyloxy-l6u-ethyl4-pregnene- 17a,21-diol-3,20-dione 2l-phosphate 6) 6a-methyl-9a-chloro-1 1 [3-tosyloxy-4-pregnene- 17 a,2l-diO1-3,20-di0116 21-phosphate EXAMPLE 1 l 9a-chlor0-1lfi-azido-4-pregnene-l7a,2l-diol-3,20-diones A. 90: chloro 115 azido 4 pregnene 1701,21- diol-3,20-diones as the 2l-phosphate.In a manner similar to that described in Example 4A treat each of the 9a chloro 11,3 tosyloxypregnane 21 phosphate products of Example 3D (i.e. compounds 3D-1 to 3D-6) with sodium azide in Water. Isolate the resultant respective products in a manner similar to that described in Example 4A to obtain the following respective products:

(1 9a-chloro-l lfl-azido-l,4-pregnadiene-l7u,2l-diol- 3,20-dione 21-phosphate (2) 9a-chloro-1I s-azido-16,8-methyl-1,4-pregnadienel7a,2l-diol-3,20-dione 2l-phosphate (3 6a-methyl-9a-chloro-l lfi-azido-lA-pregnadiene- 17a,21-diol-3,20-dione 2l-phosphate (4) 2a-methyl-9a-chloro-l1fi-azido-4-pregnene-17u,21-

diol-3,20-dione 21-phosphate (4) 2a-methyl-9u-chloro-1lB-azido-4-pregnene-17,21-

diol-3,20-dione 2l-phosphate (5 9a-chloro-1l/S-azido-l6a-ethyl-4-pregnene-1711,21-

diol-3,20-dione 2l-phosphate (6) 6u-methyl-9a-chloro-ll 3-azido-4-pregnene- 17a,21-diol-3,20-dione 21-phosphate B. 90: chloro 115 azido 4 pregnene 170;,21- diol-3,20-diones.In a manner similar to that described in Example 4B treat each of the 9a-chloro-l15-azidopregnane 21-phosphate esters of Example 11A (i.e. products 1lA-1-11A6) in solution in trishydroxymethylaminomethane at pH 8 with bacterial alkaline phosphatase. Isolate and purify the resultant respective products in a manner similar to that described in Example 4B to obtain the following respective products:

(1) 9a-chloro-l 11B-azido-l,4-pregnadiene-l7a,21-

diol-3,20-dione (2) 9a-chloro-1lB-azido-l6,6-methyl-1,4-pregnadiene- 17 a,21-diol-3,20-dione (3 6a-methyl-9a-chlor0-11,8-azido-1,4-pregnadiene 17a,21-diol-3,20-dione (4) 2a-methyl-9a-chloro-1 1,8-azido-4-pregnene-l70:,21-

diol-3,20-dione (5 9a-chloro-1lfi-azido-l6a-ethyl-4-preguene-17a,2l-

diol-3,20-dione (6) 6a-methyl-9a-chloro-11,6-azido-4-pregnene-170;,21-

diol-3 ,20-dione C. chloro 1lB-azido-4-pregnene-17u,2l-diol-3,20 dione 2l-acetates.In a manner similar to that described in Example 4C treat each of the 9oL-ChlO10-11fiaZldO-21- hydroxypregnanes of Example 11B (i.e. products 11B-lllB-6) with acetic anhydride in pyridine. Isolate and purify the resultant product in a manner similar to that described to obtain respectively:

(10) 6u-fiuoro-9a-chloro-1lfl-tosyloxy-l,4-pregnadien- 21-o1-3,20-dione 21-phosphate (1 1) 6a, 17a-dimethyl-9u-ch1oro-1 1 3-tosyloxy-4- pregnen-21-ol3,20-dione 21-phosphate (12) 6a-fluoro-9u-chloro-1 1 fi-tosyloxy- 17 a-acetoxy- 4-pregnen-21-ol-3,20-dione 21-phosphate (13) 6a-fluoro-9u-chl0ro-1 1B-tosyl0xy-17a-methyl- 4-pregnen-21-ol-3,20-di0ne 21-phosphate (14) 6m,17a-dirnethyl-9ot-chloro-l 1;3-tosy1oxy-1,4-

pregnadien-Z1-ol-3,20-di0ne 2 l-phosphate C. 90: chloro 11,9 azido 4 pregnen 21 01- 3,20 dione 21 -phosphates.-In a manner similar to that described in Example 4A treat each of the 9a-chloro- 11,8 tosyloxy 21 hydroxypregnane 21 phosphate intermediates prepared in Example 14B with sodium azide in water. Isolate the resultant respective products in a manner similar to that described to obtain the following respective products:

(1) 9a-chloro-11 3-azid0-4-pregnen-21-ol-3,20-di0ne 21-phosphate (2) 9a-chloro-1 lfi-azido-1,4-pregnadien-21-ol-3,20-

dione 21-phosphate 3) 9a-chloro-1lfl-azido-19-nor-4-pregnen-21-ol-3,20-

dione 21-phosphate (4) 6a-methyl-9a-chloro-11p-azido-4-pregnen-2l-ol- 3,20-dione 21-phosphate 5) 9a-chloro-1 1/3-azido-17a-acetoxy-4-pregnen-2 lol-3,20-dione 21-phosphate (6) 9a-chloro-1 lfi-azido-17a-caprooxy-4-pregnen- 21-ol-3,20-dione 21-phosphate 7) 6a-methyl-9a-chloro-11fl-azid0-l7u-acetoxy-4- pregnen-21-ol-3 ,20-dione 21-phosphate (8 9a-chloro-1 1 fi-azido-16 3-methyl-4-pregnen-2 l-ol- 3,20-dione 2l-phosphate (9) 6a-fiuoro-9a-chloro-11fl-azido-4-pregnen-21-0l-3,20-

dione 2l-phosphate 10) 6a-fiuoro-9a-chloro-1lfi-azido-1,4-pregnadien-21- ol-3,20-dione 21-phosphate (11) 6a,17a-dimethyl-9a-chloro-11fi-azido-4-pregnen- 21-ol-3,20-di0ne 2l-phosphate 13) 6a-fluoro-9a-chl0ro-1 1 fl-azido-l 7a-methyl-4- pregnen-21-ol-3,20-dione 2l-phosphate D. 90: chloro 11B azido 4 pregnen 21 01- 3,20 diones and 17 substituted derivatives thereof. In a manner similar to that described in Example 4B treat each of the pregnane-Zl-phosphate esters prepared in Example 14C with bacterial alkaline phosphatase in trishydroxymethylamino methane tritrated to pH 8. Isolated and purify the resultant respective products in a manner similar to that described to obtain respectively:

(1 9a-chloro-11,8-azido-4-pregnen-21-ol-3,20-

dione 2) 9a-chloro-1l/3-azido-L4-pregnadien-21-ol-3,20-

dione (3) 9a-chl0ro-1lfi-azido-19-n0r-4-pregnen-21-ol-3,20- dione I 4) 6a-methyl-9a-ch'loro-1 1 3-azido-4-pregnen-2 l-ol- 3 ,ZO-dione (5) 9a-chl0ro-1lB-azido-17a-acetoxy-4-pregnen-21- 01-3 ,20-di0ne (6) 9u-chloro-1 lfi-azido-17ucaprooxy-4-pregnen-2 1- 01-3 ,20dione (7) 6a-methyl-9a-chloro-1 1 3-azido-17u-acet0xy-4- pregnen-21-ol-3,20-dione (8) 9a-chloro-1lfl-azido-16B-methyl-4-pregnen-2l-ol- 3,20-dione (1 0) 6a-fluoro-9a-chloro-1lfi-azido-lA-pregnadien- 21-0l-3,20-dione (11) 6a,17a-dimethyl-9a-chloro-11 3-azido-4-pregnen- 21-ol-3,20-dione 12) 6a-fiuoro-9a-chloro-11fl-azido-17a-acetoxy-4- pregnen-21-ol-3,20-dione 13) 6a-fluoro-9a-chloro-1 1B-azido-17u-methyl-4- pregnen-21-ol-3,20-di0ne 14) 6a,17a-dimethyl-9a-chloro-11,B-azid0-1,4-

pregnadien-Z1-ol-3,20-dione.

E. 9a-chloro-11/8-azido-4-pregnen-21-0l-3,20-dione 21- acetate.In a manner similar to that described in Example 4C treat each of the 9u-chloro-1lfl-azido-Zl-hydroxy- 4-pregnene-3,20-diones prepared in Example 14D with acetic anhydride in pyridine. Isolate and purify the resultant respective products'in a manner similar to that described to obtain respectively:

(1) 9a-chloro-11/3-azido-4-pregnen-21-0l-3,20-dione 21- acetate (2) 9u-chloro-1 lfl-azido-1,4-pregnadien-21-ol-3,20-dione In the procedure in this example by substituting for acetic anhydride the acid anhydride of other lower alkanoic acids, e.g. by propionic acid anhydride, by caproic acid anhydride or by valeric acid anhydride, there is obtained the corresponding 21-lower alkanoate esters, e.g. the 21-propionate, the 21-caproate and the 21-valerate of the 21-hydroxy compound prepared in Example 14D.

EXAMPLE l5 A. bromo 11(3 azido 16cc methyl 1,4 pregnadiene :,21 diol 3,20 dione 21 acetate.To a solution of 4 g. of 16a-methyl-1,4,9(11)-pregnatriene l7oz,21dlOl-3,20dl0116 21-acetate and 10 g. of sodium azide in 40 ml. of acetic acid and 10 ml. water, add portion-wise over a period of 1 hour 1.3 g. of N-bromoacetamide. Dilute the reaction mixture with 20 ml. of acetic acid, then add 10 g. of sodium azide. Over an additional 1 hour period add a second 1.3 portion of N- bromoacetamide. Stir the reaction mixture until it tests negative to starch iodide paper, then pour into 300 ml. of cold water and filter. Purify the column chromatography on silica gel eluting with 50-100% ether in hexane mixture. Distil the combined eluates to a residue comprising 90: bromo llfi azido 16a methyl 1,4 pregnadiene 1711,21 diol 3,20 dione 21 acetate.

In a similar manner treat each of the following 9(11)- dehydro steroids with sodium azide and N-bromoacetamide in acetic acid:

( 1) 16a-methyl-1,4,9(11)-pregnatriene-17a,21-dio1-3,20-

dione 21-cathylate (2) 1,4,9 1 1 )-pregnatriene-l7a,21-diol-3,20-dione 21- acetate (3) 1,4,9 1 1 )-pregnatriene-16a,17u,21-triol-3,20-di0ne 16,21-diacetate (4) 16fl-methyl-l,4,9( l1)-pregnatriene-l7ix,21-diol-3,20-

dione 21-acetate (5) 6a-methyl-1,4,9(11)-pregnatriene-17a,2l-diol-3,20-

dione 21-acetate 6) 2a-methyl-4,9(11)-pregnadiene-17u,21-diol-3,20-

dione 21-acetate (7) 1,4,9(11)-pregnatriene-17a,21-diol-3,20-dione 21- cathylate Isolate and purify the resultant respective products in a manner similar to that described above to obtain respectively:

( 1) 9a-bromo-l lfi-azido-la-methyl-l,4-pregnadienel7u,2l-diol-3,20-dione Zl-cathylate (2) 9a-bromo-l lfi-azido-1,4-pregnadiene-17a,21-dio1- 3,20-dione ZI-acetate (3) 9a-bIOII10-1 lfl-azido-l,4-pregnadiene-16u,17a,2 1-

triol-3,20-dione 16,21-diacetate 4) 9a-bromo-l lfl-azido-16fi-methyl-1,4-pregnadiene- 17a,2l-dl()13,20-di011e 21-acetate (5) 6a-methyl-9a-bromo-1 lfi-azido-1,4-pregnadiene- 170:,2141101-120-(110116 21-acetate (6) 2u-methyl-9a-bromo-11B-azido-4-pregnene-17a,21-

diol-3,20-dione 21-acetate (7) 9a-bromo-1 lfi-azido-1,4-pregnadiene-l7a,21-diol- 3,20-dione 21-cathylate B. 90: bromo 11,3 azido 16a methyl 1,4 pregnadiene 170:,21 diol 3,20 dione.In a manner similar to that described in Example 1B treat 9abromo 115 azido 161x methyl 1,4 pregnadiene 1741,21 diol 3,20 dione 21 acetate with 70% perchloric acid in methanol and chloroform solution. Isolate the resultant product in a manner similar to that described to obtain 90: bromo 11,8 azido 16oz methyl 1,4 -pregnadiene 1711,21 diol 3,20 dione.

In a similar manner hydrolyze the 21-esters of each of the following prepared as described in the second paragraph of Example 15A to obtain respectively:

( 1 9'a-bromo-l lfl-azido- 1 Ga-IA-pregnadiene- 17a,2l-diol-3,20-dione (2) 9a-bromo-1lp-azido-l,4-pregnadien-17a,2l-diol- 3,20dione (3) 9a-bromo-11fi-azido-1,4-pregnadiene- 16a,17a,21-triol-3,20-dione (4) 9a-bromo-l lfi-azido-l 6,8-methyl-1,4-pregnadiene- 17a,21-di01-3,20-di01'16 (5) 6a-methyl-9a-bromo-1 l ,B-azido-1,4-pregnadiene- 17a,21-di01-3,20-di0118 6 2a-methyl-9a-bromo-1 1,8-azido-4-pregnene- 17a,21-diol-3,20-dione (7) 9a-bromo-llfi-azido-1,4-pregnadiene-17a,21

diol-3,20-dione EXAMPLE 16 9a-bromo-1lfi-azido-androstanes and pregnanes In a manner similar to that described in Example 15A, treat l7ix-acetoxy-4,9(11)-pregnadiene-3,20-dione with sodium azide in aqueous acetic acid followed by n-bromoacetamide. Isolate and purify the resultant product in a manner similar to that described to obtain 9a-bromo- 11 B-azido-lh-acetoxy 4 pregnene-3,20-dione (9abromo-l 1 ;3azido-17a-acetoxy progesterone).

In a similar manner treat each of the following 9(11)- dehydro pregnanes and androstanes with sodium azide and n-bromoacetamide in acetic acid:

( 1 16a-methyl-17a-acetoxy-4,9(11)-pregnadiene- 3 ,ZO-dione (2 17a-hydroxy-19-nor-4,9(11)-pragnadiene- 3 ,ZO-dione 26 3) 17a-hydroxy-21-fluoro-4,9(l1)-pregnadiene- 3,20-dione 4) 1,4,9(11)-androstatrien-17,8-01-3-one (5) 17u-methyl-4,9(11)-pregnadien-175-ol-3-one (6) 19-nor-4,9( 11 )-androstadiene-3,17-dione (7) 4,9(11 )-androstadiene-3,17-dione Isolate and purify the resultant respective products in a manner similar to that described above to obtain respectively,

(2) 9u-bromo-1lfl-azido-lh-hydroxy-19-norprogesterone (3) 9a-bromo-11,8-azido17a-hydroxy-21- fluoroprogesterone 4) 9a-bromo-11,B-azido-1,4-androstadien-17B-ol-3-0ne (5) 9a-bromo-1 lfi-azido- 1 7a-methyl-4-androsten- 17,B-ol-3one (6 9a-bromo- 1 lfi-azido-17a-ethinyl-4-androsten- 17,8-ol-3-one (7 9a-bromo-11,8-azido-19-nor-4-androstene- 3,17-dione (8) 9a-bromo-l lfi-azido-4-androstene-3 ,17-dione.

In a similar manner each one of the 9(11)-dehydro starting steroids listed in the foregoing Examples 1 through 15, upon treatment with sodium azide and nbromoacetamide in acetic acid in the above described manner, will yield the corresponding 9a-br0mo-l1fl-azido derivative.

EXAMPLE 17 Preparation of 9(11)-dehydro androstane and pregnane intermediates A. 16a-methyl-1,4,9(11)-pregnatriene 17a,21 diol- 3,20-dione-17,21-dipropionate and the l6 8-methyland 16-desmethyl-analogs thereof.(1) Reflux a solultion of 5 g. of 16a-methyl-1,4-pregnadiene-l15,17a,21-triol-3,20- dione in 1.3 liters of benzene under anhydrous conditions with a Dean-Stark tube. After taking off 165 ml. of benzene add 1.65 molar equivalents of triethyl-orthopropionate followed by about 0.1 molar equivalents of ptoluenesulfonic acid monohydrate. Heat at reflux temperature under a Dean-Stark tube for two hours longer, then cool to room temperature and add 5 g. of sodium bicarbonate and 5 m1. of pyridine. Concentrate the reaction mixture in vacuo to a residue. Triturate the residue with hexane-ethyl ether and filter the resultant precipitate comprising l6a-methyl-1,4-pregnadiene-11,B,17a,21 triol-3, 20-dione 17,21-methyl orthopropionate which is used without further purification in the step immediately following.

(2) Add 3 g. of 16a-methyl-1,4-pregnadiene-115,170, 21-tri0l-3,20-di0ne 17,21-methyl orthopropionate to m1. of acetic acid containing 0.9 ml. of water. Stir the reaction mixture at room temperature for about 18 hours, then pour into 450 ml. of water and filter the resultant product comprising 16a-methyl-1,4-pregnadiene-115,17, 21-triol-3,20-dione l7-propionate. Purify by recrystallization from acetone-hexane.

(3) Add 1 g. of 16a-methyl-1,4-pregnadiene-115,1712, 21-triol-3,20-dione 17-propionate to a solution of 5 ml. pyridine and 1 ml. of propionic anhydride. Allow the reaction mixture to stand at room temperature for 18 hours, then dilute with water and extract with methylene chloride. Evaporate the combined methylene chloride eluates to a residue comprising 16umethyl-1,4-pregnadiene-11p, 17a,21-triol-3,20-dione 17,21-dipropionate.

(4) To a solution of 1 g. of 16u-methyl-I,4-pregnadi ene-l15,17a,21-triol-3,20-dione 17,21-dipropionate in 5 ml. of distilled dimethylformamide, and 5 g. of anhydrous pyridine, add 0.5 ml. of methanesulfonyl chloride and heat the mixture at about C. for one hour. Cool the solution, pour into ice water and filter the resultant precipitate. After drying precipitate, dissolve it in acetone and treat the acetone solution with decolorizing carbon. Evaporate the pure acetone solution to a residue and chromatograph the residue on silica gel eluting with increasing amounts of ether-in-hexane. Combine the like fractions as determined by thin layer and spectroscopic data. Evaporate the combined eluates in vacuo to a residue comprising l6a-methyl-1,4,9(1l)-pregnatriene 17a,21 diol-3,20-dione 17,2l-dipropionate.

In a similar manner, by going through a sequence of reactions similar to that described in Procedure 17A-1 to 17A-4 by utilizing as starting compounds each of 16,8- methyl 1,4 pregnadiene-l1B,17a,2l-triol-3,20-dione and 1,4,9(11)-pregnatriene-l7a,21-diol-3,20-dione, there is obtained respectively 16 3 methyl l,4,9(ll)-pregnatrienel7a,21-diol-3,20-dione 17,2l-propionate and 1,4,9(11)- pregnatriene-17a,21-diol-3,20-dion 17,2-l-dipropio11ate.

B. 16x-methyl-1,4,9 1.1)-pregnatriene-l7a,2l-diol-3,20- dione l7-valerate 2l-acetate and the l6 8-methyl and 16- desmethyl analogs thereof.In a manner similar to that described in Example 7A (procedure 1 through 4), treat 1 d-I1'lethy1 1,4 pregnadiene-l.1fl,l7a,2l-triol-3,20-dione with trimethyl orthovalerate and p-toluenesulfonic acid monohydrate to obtain l6a-methyl-1,4-pregnadiene-1118,- l7a,2l-triol3,20-dione 17,2l-methyl orthovalerate which, upon treatment with aqueous acetic acid at room temperature in the manner of procedure .l7A2 yields 160cmethyl 1,4 pregnadiene-l118, 17a,2=l-triol-3,20-dione l7- valerate. Esterification of the 17-monovalerate thereby obtained with acetic anhydride in pyridine in a manner similar to that described in Example 17A-3 yields 16amethyl 1,4 pregnadiene-l-lfl,17a,2Jl-triol-3,20-dione 17- valerate 2l-acetate which in turn, upon treatment with methanesulfonyl chloride and pyridine in dimethylform amide is converted to l6ot-methyl-1,4,9(1l)-pregnatriene- 17o,2 l-diol-3,20-dione '17-valerate ZI-acetate.

Similarly, 16,B-methyl-l,4-pregnadiene-l 1B, 1 7oc,2 l-triol- 3,20-dione and 1,4-pregnadiene 11B,l7a,2l triol-3,20- dione are each subjected to the sequence of reactions similar to that described above and there is obtained respectively, 16,9 methyl 1,4,9(11)-pregnatriene-17a,2.ldiol-3,20-dione l7-valerate 21-acetate and 1,4,9(ll)-pregnatriene-17u,21-dio1-3,20-dione 17-valerate 2l-acetate.

C. 6,9 l l -bis-dehydro-pregnadiene intermediate-To a solution of 1 g. of 6-chl0ro-16u-methyl-hydrocortisone 2l-acetate in ml. of distilled dimethylformamide and 5 ml. of anhydrous pyridine, add 0.5 ml. of methanesulfonyl chloride and heat the mixture at about 80 C. for one hour. Cool the solution, pour into ice water and filter the resultant precipitate. After drying the precipitate, dissolve it in acetone and treat the acetone solution with decolorizing carbon. Evaporate the pure acetone solution to a residue. Chromatograph on silica gel eluting with increasing amounts of ether-in-hexane. Combine the like fractions as determined by thin layer and spectroscopic data. Evaporate the combined eluates in vacuo to a residue comprising 6-chloro-l-6u-methyl-4,6,9(l1)-pregnatriene-l7,21-diol-3,20-dione Zl-acetate.

In a similar manner treat each of the following with methanesulfonyl chloride in dimethylformamide and pyridine.

(1) 6 chloro-16a-methyl-6-dehydroprednisolone 2l-acetate (2) fi-dehydro-hydrocortisone Zl-acetate and (3) G-dehydroprednisolone 21-acetate.

Isolated and purify the resultant respective products in a manner similar to that described above to obtain respectively,

( 1) 6-Ch1OIO-16OL-m6thy1-1,4,6,9( 1 1)-pregnatriene- 17a,21-diOl-3,20-dl01'l6 2l-acetate (2) 4,6,9 l l )-pregnatriene-l7a,2 ldiol-3,20-dione Zl-acetate and (3) 1,4,6,9( 11)-pregnatetraene l7a,21-diol-3,20-dione 2l-acetate.

D. 9(11) dehydrotestosterone intermediates-Utilizing known techniques, submit each of the following testosterone derivatives to the action of Curvularia lunata (N.R.R.L. 2380):

( l) l7a-methyltestosterone (2) 17e-ethyltestosterone (3) l7a-vinyltestosterone and (4) l7a-ethinyltestosterone.

Isolate the resultant respective products according to known techniques to obtain respectively,

( 1 1 lB-hydroxy-l7u-methyltestosterone (2) l lp-hydroxyl 7a-ethyltestosterone (3) llfi-hydroxy-l7u-vinyltestosterone and (4) 1 lfl-hydroxy-l7a-ethinyltestosterone.

Treat each of the foregaoing llfi-hydroxy compounds with methanesulfonyl chloride in pyridine and dimethylformamide in a manner similar to that described in above Example to obtain respectively,

( l 17a-methyl-9(l l)-dehydrotestosterone 2) 17a-ethyl-9(l l)-dehydrotestosterone (3 17a-vinyl-9(1 l)-dehydrotestosterone and (4) 17.a-ethinyl-9(l l)-dehydrotestosterone.

E. 6 chloro 17a-acetoxy 9(11) dehydroprogesterones.( 1 6a-chloro-17a-acetoxy-9(1l)-dehydroprogesterone is obtained from 17a-acet0xy-9(l1)-dehydroprogesterone by conversion thereof to the corresponding ethyl enol ether by treatment with ethyl orthtoformate in dioxane according to known techniques followed. by treat ment of the 3-ethoxy-l7a-acetoxy-3,5,9(1l)-pregnatriene- 20-one thereby formed with N-chlorosuccinimide in acetone and aqueous acetic acid in the presence of sodium acetate to obtain 6,3-chloro-17a-acetoxy-4,9(11)-pregnadiene-3,20-dione. Treatment of the foregoing oft-chloro compound with acid in known manner yields 6u-chloro- 17a acetoxy-4,9(1l)-pregnadiene-3,20-dione (Ga-ChlOrO- l7a-'acetoxy-9 l l -dehydroprogesterone (2) Heat at reflux temperature for 13 hours under an atmosphere of nitrogen, a mixture of 2 g. of out-methyl- 16-methylene-17a-acetoxy-4, 9 1 1 )-pregnadiene-3,20-dione and 5 g. of chlorinil in 60 ml. of ethyl acetate and 15 m1. of acetic acid. Evaporate the reaction mixture to a residue and extract with ethyl acetate. Combine the organic extracts and wash with cold 1% aqueous sodium hydroxide, then with water and dry over magnesium sulfate. Filter and evaporate to a residue comprising 6-chloro l 6-methylene-17a-acetoxy-4,6,9( 1 1 -pregnatriene-3,20- dione (6 chloro-l6-methylene-17a-acetoxy-6,9,(l1)-bisdehydroprogesterone). Purify by crystallization from acetone-hexane.

In a similar manner treat 6a-chloro-l7a-acetoxy- 4,9(l1)-pregnadiene-3,20-dione with chlorinil. Isolate and purify the resultant product in a similar manner to obtain 6-chloro-17a-acetoxy-4,6,9( 11 )-pregnatriene-3,20-dione (3) Stir a reflux temperature for 3 hours a mixture of 19.5 g. of 6a-chloro-l6-methylene-17a-acetoxy-4,9(l1)- pregnadiene3,20-dione in ml. of dioxane and 19.5 g. of dichlorodicyanobenzoquinone. Cool to room temperature, filter and dilute the filtrate with 2 liters of meth ylene chloride, wash the methylene chloride solution with 1% aqueous sodium hydroxide and then with water until the water extracts are neutral. Dry the methylene chloride solution over sodium sulfate, filter and evaporate in vacuo to a residue comprising 6a-chloro-16-methylene-17aacetoxy-1,4,9( l l -pregnatriene-3,20-dione.

In a similar manner treat 6a-methyl-17a-acetoxy-4,9( 1 l )-pregnadiene-3,20-dione, 6-chloro-1-methylene-17a-acetoxy-4,6,9(11)- pregnatriene-3,20-dione and 6-chloro-l7a-acetoxy-4,6,9(1 1)-pregnatriene3,20-dione with dichlorodicyanobenzoquinone to obtain respectively,

( l 6a-methyll7u-acetoxy-l ,4,9( 1 1 -pregnatriene- 3 ,20-dione (2) 6-chloro-l6-methylene-17a-acetoxy-l,4,6,9( l1)- pregnatetraene-3,ZO-dione and 29 (3) 6-chloro-l7a-acetoxy-l,4,6,9(11)pregnatetraene- 3,20-dione.

EXAMPLE 18 9a-chloro-1lfl-azido-4-pregnene-17a,21-diol-3,20- diones and esters thereof 7: 9a-chloro 11,B-azido-16a-metl1yl-17a,21-diol-3,20- dione 17,2l-dipropionate.In a manner similar to that described in Example 1A, treat 16a-methyl-1,4,9(11)- pregnatriene-17u,21-dio1-3,20-dione 17,21 dipropionate with sodium azide in aqueous acetic acid followed by treatment with N-chlorosuccinimide. Isolate and purify the resultant product in a manner similar to that described to obtain 9a-chloro-115-azido-16a methyl 1,4-pregnadiene-17a,2l-diol-3,20-dione 17,21-diprpionate.

In a similar manner treat each of the following with sodium' azide in aqueous acetic acid followed by N- chlorosuccinimide:

(1) 16a-methyl-1 ,4,9(1 l)-pregnatriene- 17a,2 1-diol-3,20-

dione 17,21-dipropionate (2) 1,4,9 1 l)-pregnatrienel7a,2 l-diol3,20-dione 17,21-

dipropionate (3) 16u-methyl-l,4,9(11)-pregn-atriene-17a,21-diol-3,20-

dione -17-valerate 21-acetate (4) 16,8-methyl-1,4,9(11)-pregnatriene-17a,21-diol-3,20-

dione 17-valerate ZI-acetate (5) 1,4,9(11)-pregnatriene-17a,21-diol-3,20-dione 17- valerate 2 1 -acetate (6) 6-chloro-16a-methyl-4,6,9(l1)-pregnatriene-17a,21-

diol-3;20-dione 17-valerate 21-acetate (7) 6-chloro-16a-methyl-1,4,6,9(1 1)-pregnatetraene- 17a,2l -diol-3,20-dione 2l-acetate (8) 6-chloro-4,6,9(11)-pregnatriene-17a,21-di0l-3,20-

dione 2l-acetate and (9) 6-chloro-1,4,6,9(11)-pregnatetraene-17u,21-diol-3,20-

dione 21-acetate.

Isolate and purify the resultant respective products in a manner similar to that described above to obtain respectively,

In the above procedure by substituting for N-chlorosuccinimide the reagent N-bromoacetarnide and carrying out the reaction in a manner similar to that described in Example A, utilizing as starting compounds the 9(11)- dehydro steroids listed in the first and second paragraphs of this example, there is obtained the corresponding 90:- bromo-llfl-azido-derivatives, i.e. 9a-bromo-11B-azido- 1 fia-methyl-1,4-pregnadiene-1711,21-diol-3,20-dione 17,21- dipropionate and the 9a-bromo analogs of the 9a-chloro products listed in the third paragraph of this example, i.e.: l) 9a-bromo-1lfi-azido-l6/3-rnethyl-1,4-pregnadiene- 17a,21-diol3,20-dione 17,21-dipropionate (2) 9a-bromo-1 lfi-azido-1,4-pregnadiene-1711,2l-diol- 3,20-dione 17-,2-dipropionate (3 9a-bromo-1lfi-azido-16u-methyl-1,4-pregnadiene- 17a,21-di0l3,20-di0116 17-valerate 21-acetate 4) 9a-bromo-1IB-azido-16,8-methyl-1,4-pregnadiene- 17a,2 1-diol-3,20-dione 17-valerate 2 l-acetate (5 9a-bromo-1 lfi-azido-1,4-pregnadiene-l7a,2l-di0l 3,20-dione 17-valerate 21-acetate (6) 6-chloro-9a-bromo-1 1B-azido-16a-methyl-4,6- pregnadiene-l7a,21-diol-3,20-dione 17-valerate 2 l-acetate 7) 6-Ch1OIO-9a-brOInO-l lfi-azido-l6a-methyl-1,4,6- pregnatriene-l7a,2l-diol-3,20-dione 21acetate (8) 6-chloro-9wbromo-1 1 fi-azido-4,6-pre gnadiene-17a,21-

diol-3,20-dione ZI-acetate and (9) 6-ChlOIO-9a-bIOIHO-l lfi azido-1,4,6-pregnatriene- 17a,2l-diol-3,20-dione 21-acetate.

B. chloro 11,8 azido I6tx-methyl-L4-pregnadiene-17a,2l-diol 3,20 dione 17 propionate.-Add 1 g. of 9oc-Chl0r0-l lp-azido-l6u-methyl-1,4-pregnadiene- 17a,21-diol-3,20-dione 17,21-dipropionate to a solution of 7 ml. of 70% aqueous perchloric acid in 68 ml. of methanol maintained at about 0 C. Stir the reaction mixture for about 48 hours, then pour into 700 ml. of cold Water. Filter the resultant precipitate, air dry at 40 C. and purify by crystallization from acetone-hexane to obtain 90:- chloro 11B azido 16oz methyl 1,4 pregnadiene- 17a,21-diol-3,20-dione 17-propi0nate.

In a manner similar to that described above, treat each of the 90: chloro-11[3-azido-4-pregnene-17u,21-diol-3,20- dione 2l-esters and the corresponding 9oc-bromo-analogs prepared as described in Example 18A with 70% perchloric acid in methanol. Isolate and purify the resultant respective products in a manner similar to that described to obtain the corresponding 2l-hydroxy compounds, i.e. respectively.

( 1 9a-chloro-1lfi-azido-l6 3-methyl-1,4-pregnadiene- 17a,21-diol-3,20-dione l7-propionate (2) 9a-chloro-1 1 fl-azido-1,4-pregnadiene-17a,21-diol- 3,20-dione 17-propionate (3) 9a-ch1oro-1lfl-azido-16a-methyl-1,4-pregnadiene- 17a,21-diol-3,20-dione 17-valerate (4) '9a-ChlOrO-l lfi-azido-l6 3-methyl-1,4pregnadiene- 17oc,21-di0l-3,20-dl0l16 17-valerate (5) 9a-chloro-1 1,8-azido-1,4-pregnadie'ne-170:,21-di0l- 3,20dione 17-valerate 6) 6,9u-dichl0ro-1lfl-azido-16a-methyl-4,6-pregnadiene- 17a,2l-diol-3,20-dione 17-valerate (7) 6,9a-diChl01O-1 lfl-azido-l6a-rnethyl-1,4,6-

pregnatriene-17a,21-diol-3,20-dione (8) 6,9a-dichloro-11B-azido-4,6-pregnadiene-17u,21-

diol-3,20-dione and (9) 6,9a-dichloro-1 lfi-azido-l,4,6-pregnatriene-l7a,2 1-

diol-3,20-dione.

EXAMPLE 19 9a-chl0ro-1lB-azido-progestero'nes and the corresponding 9tx-bromo-l 1 B-azido-derivatives In a manner similar to that described in Example 1A, treat each of the following 9 1 1 -dehydro-steroids with sodium azide in aqueous acetic acid followed by N-chlorosuccinimide:

(1) 6a-chloro-17a-acetoxy-4,9(11)-pregnadiene-3,20-

dione (2) 6-chlor0-17a-acet0Xy-4,6,9(11)-pregnatriene- 3,20-dione (3) 6-chloro-l7a-acetoxy-1,4,6,9(l1)-pregnatriene- 3,20-dione 4) 6oz-ChlOIO-17oc-3CB1ZOXY-1 ,4,9 l 1)-pregnatriene- 3,20-dione (5 6a-chloro16-methylene-17a-acetoxy-1,4,9(11)- pregnatriene-3,20-dione 6) 6-chloro-16-methylene-17a-acetoXy-1,4,6,9( l1)- pregnatetraene-3,ZO-dione (7) 6-chloro-16-methylene-l7a-acetoXy-4,6,9(11)- pregnatriene-3,20-dione 8) 4,9(11)-androstadiene-3,17-dione (9) 17a-methyl-4,9( 11)-androstadien-1718-ol-3-one 3 1 (10) 17a-methyl-4,9(11)-androstadien-17,6-01-3-one 17-propionate (11) 17a-ethyl-4,9(11)-androstadien-17,8-ol3-one (12) 17u-vinyl-4,9(1l)-andr0stadien-17,B-ol-3-one (13) 4,9(11)-pregnadiene-3,20-dione (14) 17ot-acetoxy-4,9(11)-pregnadiene-3,20-dione (15) 6a-fluoro-l7a-acetoxy-4,9(11)-pregnadiene- 3,20-dione.

Isolate and purify the resultant respective products in a manner similar to that described in Example 1A to obtain respectively,

( 1) 6a,9a-dichloro-1lB-azido-17a-acetoxy-4-pregnene- 3,20-dione (2) 6,9a-dichloro-1lfl-azido-17a-acetoxy-4,6-

pregnadiene-3,20-dione (3 6,9a-dichloro-l lfi-azido- 1 7a-acetoxyl ,4,6-

pregnatriene-3,2()-dione (4) 60,9oL-diChlOTO-1 1 fl-azido-lh-acetoxy- 1,4-

pregnadiene 3,20-dione (5) 6a,9a-dichloro-11/3-azido-16-methylene-17aacetoxy-1,4-pregnadiene-3,20-dione (6) 6,9u-dichloro-1 1 ,B-azido-16-methylene-17a-acetoxy- 1,4,6-pregnatriene-3,ZO-dione (7) 6,'9oc-diChl01'O-1 1 B-azido-16-methylene-17a-acetoxy- 4,6-pregnadiene-3,20-dione (8) 9a-Ch1OI'O-1 1 ,8-azido-4-androstene-3, 17-dione (9) 9a-Chl0I'O-1 1,8-azido-17u-methyl-4-androstenel7 8-ol-3-one (10) 9tx-chloro-1lfi-azido-17a-methyl-4-androsten- 1 75-01-3 -one 17-propionate (l 1 9a-ch1oro-1 1 fl-azido-17a-ethyl-4-androsten-17,6-

ol-3-one 12) 90t-Ch1OI'O-1 1fl-azido-4-pregnene-3 ,20-dione (13) 9a-chlor0-1 1 p-azido- 17a-acetoxy-4-pregnene-3,20-

dione and (14) 6oc-flLIOrO-a-ChlO1'O-1 1fi-azido-17a-acetoxy-4- pregnene-3,20-dione.

B. Treat each of the 9(11)-dehydro steroid starting compounds listed in Example 19A with sodium azide in aqueous acetic acid followed by N-bromoacetamide in a manner similar to that described in Example 15A. Isolate and purify the resultant respective products to obtain respectively:

(1 6ot-chloro-9a-bromo-11 8-azido-17u-acetoxy-4- pregnene-3,20-dione (2) 6-chloro-9a-brorno-1lfi-azido-17a-acetoxy-4,6-

pregnadiene-3,20-dione (3 6-chloro-9a-bromo-11 8-azido-17a-acetoxy-1,4,6-

pregnatriene-3,20-dione (4) 6oc-Ch101'0-9oc-bIOII10-1 1,8-azido-17u-acet0xy-1,4-

pregnadiene-3,20-dione 5) 6a-chloro-9a-bromo-1 1[3azido-16-methylene-17:x-

acetoxy-1,4-pregnadiene-3,ZO-dione (6) 6-chloro-9tx-bromo-1lfl-azido-l6-methylene-17otacetoxy-1,4,6-pregnatriene-3,ZO-dione (7) 6-chloro-9a-br0mo-1 1 fi-azido-16-methylene-17aacetoxy-4,6-pregnadiene-3,20-dione (8) 9a-bromo-11fi-azido-4-androstene-3 ,17-dione (9) 9a-bromo-11fl-azido-17a-methyl-4-androsten- 17fi-ol-3-one 1 1) 9u-bromo-1I/S-azido-17a-ethy1-4-androsten-17-01- 3-one (12) 9a-bromo-1 1 B-azido-4-pregnene-3 ,ZO-dione 13) 9a-bromo-1 1 fi-azido-17a-acetoxy-4-pregnene- 3,20-dione and EXAMPLE 20 9a bromo 11 3 azido 4 pregnene 1701,21 diol 3,

ZU-dione (prepared via the replacement of the 115- tosyloxy group (azid0)) A. 9ot-bromo-11 3-tosyloxy-16a-methyl-1,4-pregnadiene 17a,21-diol-3,20-dione 21-phosphate.Treat 16a-methyll,4,9(11)-pregnatriene 1701,21 diol-3,20-dione 21-ph0sphate (the product of Example 3C) in a manner similar to that described in Example 3D by utilizing in place of N-chlorosuccinimide the reagent N-bromoacetamide. Isolate and purify the resultant product in a manner similar to that described in Example 3D to obtain 9u-bI0II10-11fitosyloxy 16a methyl 1,4 pregnadiene 17a,21 diol- 3,20-dione 21-phosphate.

Similarly, 6-chloro-4,6,9(11)-pregnatriene-17a,2l-diol- 3,20-dione 21-phosphate (prepared from the corresponding 21-hydroxy compound in the manner of Examples 3A-3C), and 6-chloro-1,4,6,9(11)-pregnatetraene-l7a,2ldiol 3,20 dione 21 phosphate, when reacted with p-toluenesulfonic acid and N-bromoacetamide in the manner of Example 20A, there is obtained 6-chloro-9a-bromo- 11B tosyloxy 4,6 pregnadiene 170:,21 diol 3,20- dione 21-phosphate and 6-ChlO1O-9cz-bIOIHO-1IB-tOSYlOXY- 1,4,6-pregnatriene-17a,21-diol-3,20-dione 21-phosphate.

B. 9a-bIOII10-11B-a2idO-16a methyl 1,4 pregnadiene- 17a,21 1iol-3,20-dione '21-phosphate.-In a manner similar to that described in Example 4A, treat Qa-bI'OIIlO-l 1B- tosyloxy 16a methyl 1,4 pregnadiene 1711,21 diol- 3,20-dione 21-phosphate with sodium azide in water. Isolate and purify the resultant product in a manner similar to that described to obtain 9a-bl'OmO-11B-3ZidO-l6ozmethyl 1,4 pregnadiene 17a,21 diol 3,20 dione 21-phosphate.

Similarly, treat 6-chloro-9a-bromo-11,8-tosyloxy-4,6- pregnadiene-17a,2l-diol-3,20-dione 21-phosphate and 6- chloro c bromo tosyloxy 1,4,6 pregnatriene- 17a,21-diol-3,20-dione 21-phosphate with sodium azide in water to obtain respectively,

(1 6-chloro-9a-bromo-1l/i-azido-4,6-pregnadiencl7a,21-diol-3,20-dione 2l-phosphate and (2) 6-Ch10l'O-90t-bIOmO-1 IB-azido-l,4,6-pregnatriene- 17a,21-diol-3,20-dione 21-phosphate.

C. 9a-bromo-11B-azido-16a methyl 1,4 pregnadiene- 17a,21-diol-3,20-dione.ln a manner similar to that described in Example 48, treat each of the 21-phosphate products prepared in Example 208 with bacterial alkaline phosphatase. Isolate and purify the resultant respect products in a manner similar to that described to obtain respectively,

(1) 9a-bromo-1lfl-azido-l6a-methyl-1,4-pregnadiene- 17a,21-diol 3,20-dione (2) 6-chloro-9a-bromo 11B-azido-4,6-pregnadiene- 17a,2 l-dio1-3,20-dione and (3) 6-chloro-9a-bromo-1lfi-azido-1,4,6-pregnatriene- 17a,21-dio1-3,20-dione.

EXAMPLE 21 6,9a-diCh10I'O-1 1fl-azido-4,6-pregnadienes prepared by the replacement of the ll-tosyloxy group by azido In a manner similar to that described in Examples 3D and 4A and 4B, treat each of 6-chloro-4,6,9(11)-pregnatriene-17a,21-diol-3,20-dione 21-phosphate and the l-dehydro analog thereof with p-toluenesulfonic acid and N-chlorosuccinimide to obtain respectively 6,9a-dichloro- 115 tosyloxy 4,6 pregnadiene :,21 diol 3,20- dione 21-phosphate and the l-dehydro analog thereof followed by treatment of the foregoing llfi-tosyloxy products with sodium azide in water to obtain respectively, 6,9a dichloro 1113 azido 4,6 pregnadiene 170;,21- diol-3,20-dione 21-phosphate and 6,9ot-dichloro-11B-azido- 1,4,6-pregnatriene-17a,21-diol-3,20-dione 21-phosphate.

Treatment of the foregoing 21-phosphate esters with bacterial alkaline phosphatase yields the corresponding 21 hydroxy compounds, namely 6,9u-dichloro-1'1flazido-4,6-pregnadiene-17u,21-diol-3,20-dione and 6,9a-di- N? -ww X Y I I wherein:

X is a halogen having an atomic weight greater than 20 and less than 100;

Y is a member selected from the group consisting of hydrogen and methyl;

W is a member selected from the group consisting of hydrogen, lower alkyl, and lower alkylidene; and

Z is a member selected from the group consisting of oxygen,

and

ooomv 1 A being a member selected from the group consisting of hydrogen, lower alkyl, and lower alkinyl; R being a member selected from the group consisting of hydrogen and lower alkanoyl; T being a member selected from the group consisting of hydrogen, hydroxy, and lower alkanoyloxy; and V being a member selected from the group consisting of hydrogen, fluorine, hydroxy, lower alkanoyloxy, lower alkoxycarboxy, and dihydroxyphosphoryloxy.

2. A l-dehydro compound according to claim 1, wherein X is a halogen having an atomic weight greater than 20 and less than 100, Y is methyl, W is hydrogen and lower alkyl, and Z is CHzVCO T being a member selected from the group consisting of hydroxy and lower alkanoyloxy; and V being a member selected from the group consisting of hydroxy, lower alkanoyloxy, ethoxycarboxy and dihydroxyphosphoryloxy;

said compound being a member selected from the group 34 consisting of 9a-X-1lfi-azido-l,4-pregnadiene-17a,2ldio1-3,20-dione and the 16-methyl analogs thereof, and their 17-lower alkanoate, 21-lower alkanoate, 17,21-dilower alkanoate, 21-carboethoxylate, and the 2l-phosphate esters.

3. 9oz chloro 11,3 azido 16a methyl 1,4- pregnadiene-17a,21-dio1-3,20-dione.

4. 9oz chloro 11,6 azido 16a methyl 1,4- pregnadiene-17a,21-di0l-3,20-dione ZI-acetate.

5. chloro azido methyl 1,4- pregnadiene-l7a,2l-diol-3,20-dione.

6. 9a chloro 115 azido 1 6 methyl-1,4-pregnadiene-17u,21-diol-3,20-dione 21-phosphate.

7. 9a chloro 11B azido '16 methyl-1,4-pregnadiene-'17,21-dio1-3,20-dione 17,21-dipropionate.

8. 9a chloro 11B azido 16 methyl-1,4-pregnadiene-17u,21-diol-3,20-dione'17-va1erate.

9. In the process for preparing a compound according to claim 1, the step which comprises treating a 9(11)- dehydro steriod selected from the group consisting of a 9(11)-dehydroandrostane and a 9(11)-dehydropregnane with an alkali metal azide and a halogenating agent selected from the group consisting of a positive chlorine donor and a positive bromine donor.

10. The process according to claim 9 wherein said alkali metal azide is sodium azide.

11. The process according to claim 9 when carried out in aqueous lower alkanoic acid.

12. The process according to claim 9 when carried out in aqueous lower alkanoic acid and wherein said alkali metal azide is sodium azide, and said halogenating agen is N-chlorosuccinimide.

13. The process according to claim 9 when carried out in aqueous lower alkanoic acid and wherein said alkali metal azide is sodium azide, said halogenating agent is N-bromacetamide.

14. The proces according to claim 9 when carried out in aqueous acetic acid and wherein said 9(11)-dehydro steroid is 1611: methyl-1,4,9(11)-pregnatriene-17u,21- dio1-3,20-dione 21-acetate, and said alkali metal azide is sodium azide, said halogenating reagent is N-chlorosuccinimide.

15. The process according to claim 14 including the step of isolating the 9ct-chloro-11fl-azido-16a-methy1-1,4 pregnadiene-17u,21-diol-3,20-di0ne 21 acetate thereby formed.

No references cited.

ELBERT L. ROBERTS, Primary Examiner US. Cl. X.R.

z gz g? UNITED STATES PATENT OFFICE CERTIFICATE OF CORRECTION Patent No. 5:h55:968 Dated July 5: 1969 Inventor(s) Hershel L. Herzog and Elliot L. Shapiro It is certified that error appears in the above-identified patent and that said Letters Patent are hereby corrected as shown below:

r- Column 1, line 52, "to process for" should read ---to processes for---. Column 7, line 52, "of 90l-halogeno-" should read ---a 9Ct-halogeno- Column 7, lines 67 and 68, the phrase "at 0-11 an azide" should read ---at (3-11 with an azide---. Column 8, line 56, the second and third words "the dilute" shoulc read ---then dilute---. Column 8, line 52, the second word "and" should read ---the---. Column 8, line 52, the phrase "is determined" should read ---as determined---. Column 8, line "-l7Cl,2l-d1ol 5,20-dione" should read -l701,2l-dio1-5,20-dion4 Column 10, line 11, "5,50-dione" should read ---5,20-dione---. Column 12, line 69, "B-tosyloxy" should read ---llB-tosyloxy---. Column 15, line 25, "2l-fethanesulfonate" should read ---2lmethanesulfonate---. Column 1 lines +0 and +1, delete the second occurrence of product (h i.e -l) 2(1-methyl-90t-chlorollfia'tido-h-pregnene-l70,2l-diol-5,20-dione 2l-phosphate". Column 15, line ho, +,9(l)pregnadiene" should read +,9(11)- pregnadiene---. Column 19, line 62, the first word "Isolated" should read ?--Isolate---. Column 20, line 52, "norr 501" should read ---nor-50t- Column 22, line 55, in the compound name replace "l9-nor with ---l66-methyl---. Column 22, line 60, "pregnadient" should read ---pregnadiene---. Column 22, line 72 "lh-pregnene" should read +-pregnene--. Column 2 line 57, "60!, 'TOt-" should read. ---6(I,170l---. Column 25, line +5, "160- l, should read ---l6Ot-methyl-l, Column 26, line 56, "5,20-dione-l7,2l-dipropionate" should read ---5,20-dione l7,2l-dipropionate---. Column 27, line 16, "dion" should read ---dione---. Column 27, line 6 "Isolated" should read --Isolate---. Column 27 line 68, "1, 56,9(ll)-pregnatriene" should read ---l, +,6,9(ll$pregnatetraene--. Column 28, line 5 "Stir a" should read ---Stir at---. Column 29, line T5, "17-,2- dipropionate" should read ---l7,2l-dipropionate---. Column 50, line 6 "l,h,6,9( ll)-pregnatriene" should read ---l, +,6,9(ll)- pregnatetraene---. Column 51, line 56, 'ob-chloro" should read ---90t-chloro---. Claim 2, column 55, lines &8 and +9, the forum I- therein should appear as follows: 2

SIGNED AND SEALED APR 2 81970 (SEAL) Attest:

E when dwardm'ne wnmnm E. 'SGHUYLER, m.

Attesting Officer Commissioner of Paton 

